Osteoprotegerin Is an αvβ3-induced, NF-κB-dependent Survival Factor for Endothelial Cells
作者： Uriel M. Malyankar , Marta Scatena , Katherine L. Suchland , Theodore J. Yun , Edward A. Clark
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摘要: Osteopontin protects endothelial cells from apoptosis induced by growth factor withdrawal. This interaction is mediated the alpha(v)beta(3) integrin and NF-kappaB-dependent (Scatena, M., Almeida, Chaisson, M. L., Fausto, N., Nicosia, R. F., Giachelli, C. (1998) J. Cell Biol. 141, 1083-1093). In present study we used differential cloning to identify osteopontin-induced, genes in cells. One of identified this screen was osteoprotegerin, a member tumor necrosis receptor superfamily. By Northern Western blot analysis, osteoprotegerin mRNA protein levels were very low plated on non-integrin cell attachment factor, poly-d-lysine. contrast, 5-7-fold following ligation osteopontin. Osteoprotegerin induction osteopontin time-dependent observed as early 3 h treatment. NF-kappaB inactivation achieved over expression an IkappaB super repressor completely inhibited Finally, purified protected with inactive deprivation. These data suggest that alpha(v)beta(3)-mediated survival depends indicate new function for
sci-hub.se 参考文章(23)
D. J. Van Antwerp, S. J. Martin, T. Kafri, D. R. Green, I. M. Verma, Suppression of TNF-α-Induced Apoptosis by NF-κB Science. ,vol. 274, pp. 787- 789 ,(1996) , 10.1126/SCIENCE.274.5288.787
J. Kimble Frazer, Maria K. Ewings, Stephen M. Schwartz, Edward A. Clark, Leroy E. Hood, Geraldine L. Shu, Preet M. Chaudhary, Theodore J. Yun, Virginia Pascual, OPG/FDCR-1, a TNF Receptor Family Member, Is Expressed in Lymphoid Cells and Is Up-Regulated by Ligating CD40 Journal of Immunology. ,vol. 161, pp. 6113- 6121 ,(1998)
A. A. Beg, D. Baltimore, An Essential Role for NF-κB in Preventing TNF-α-Induced Cell Death Science. ,vol. 274, pp. 782- 784 ,(1996) , 10.1126/SCIENCE.274.5288.782
Christiane Ferran, Deborah M Stroka, Anne Z Badrichani, Jeffrey T Cooper, Christopher J Wrighton, Miguel Soares, Shane T Grey, Fritz H Bach, None, A20 Inhibits NF-κB Activation in Endothelial Cells Without Sensitizing to Tumor Necrosis Factor–Mediated Apoptosis Blood. ,vol. 91, pp. 2249- 2258 ,(1998) , 10.1182/BLOOD.V91.7.2249
C. Wang, NF-B Antiapoptosis: Induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to Suppress Caspase-8 Activation Science. ,vol. 281, pp. 1680- 1683 ,(1998) , 10.1126/SCIENCE.281.5383.1680
Z. Zhang, K. Vuori, J. C. Reed, E. Ruoslahti, The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 6161- 6165 ,(1995) , 10.1073/PNAS.92.13.6161
WS Simonet, DL Lacey, CR Dunstan, MCMS Kelley, M-S Chang, R Lüthy, HQ Nguyen, S Wooden, L Bennett, T Boone, G Shimamoto, M DeRose, R Elliott, A Colombero, H-L Tan, G Trail, J Sullivan, E Davy, N Bucay, L Renshaw-Gegg, TM Hughes, D Hill, W Pattison, P Campbell, S Sander, G Van, J Tarpley, P Derby, RAEP Lee, WJ Boyle, None, Osteoprotegerin: A Novel Secreted Protein Involved in the Regulation of Bone Density Cell. ,vol. 89, pp. 309- 319 ,(1997) , 10.1016/S0092-8674(00)80209-3
Peter C. Brooks, Anthony M.P. Montgomery, Mauricio Rosenfeld, Ralph A. Reisfeld, Tianhua Hu, George Klier, David A. Cheresh, Integrin αvβ3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels Cell. ,vol. 79, pp. 1157- 1164 ,(1994) , 10.1016/0092-8674(94)90007-8
U.M. Malyankar, R. Hanson, S.M. Schwartz, A.L. Ridall, C.M. Giachelli, Upstream stimulatory factor 1 regulates osteopontin expression in smooth muscle cells. Experimental Cell Research. ,vol. 250, pp. 535- 547 ,(1999) , 10.1006/EXCR.1999.4537
S Strömblad, J C Becker, M Yebra, P C Brooks, D A Cheresh, Suppression of p53 activity and p21WAF1/CIP1 expression by vascular cell integrin alphaVbeta3 during angiogenesis. Journal of Clinical Investigation. ,vol. 98, pp. 426- 433 ,(1996) , 10.1172/JCI118808