Thermally reduced graphene oxide: The study and use for reagentless amperometric d-fructose biosensors
作者： Ieva Šakinytė , Jurgis Barkauskas , Justina Gaidukevič , Julija Razumienė
DOI: 10.1016/J.TALANTA.2015.07.072
关键词:
摘要: Aiming to create reagentless amperometric D-fructose biosensor, graphene based electrode materials have been synthesized by newly proposed thermal reduction of oxide. The method allowed separate and collect different fractions thermally reduced oxide (TRGO) with physicochemical properties. structural characteristics surface morphologies TRGO were evaluated using SEM, XRD, TGA analysis, Raman spectroscopy BET measurements. Three tested as for biosensors. direct electron transfer (DET) from the active site dehydrogenase (FDH) was achieved all fractions. High values sensitivity (up 14.5 μA mM(-1) cm(-2)) are same order these other sensors on synergistic mediated processes. relationships between structure molecular processes determining effect DET in bioelectrocatalysis FDH studied. Stability biosensors also assessed. best results when immobilization performed a crosslinking glutaraldehyde. For group, after period 5 days biosensor determination decreased less than 20%.
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