Effects of adsorption of benzo[a]pyrene onto carbon black particles on levels of DNA adducts in lungs of rats exposed by inhalation

作者: Ronald K. Wolff , James A. Bond , James D. Sun , Rogene F. Henderson , Jack R. Harkema

DOI: 10.1016/0041-008X(89)90334-7

关键词:

摘要: Exposure of rodents to benzo[a]pyrene (BaP) associated with particles has previously been shown result in increased retention BaP and metabolites lungs. To determine if DNA damage might be enhanced, adducts were measured lungs F344 rats following inhalation pure aerosols or absorbed on carbon black particles. Groups exposed nose only filtered air, [14C]BaP (2 mg/m3), mg/m3) adsorbed (97 (BaPCB) for 4 hr/day, 1 day/week, 12 weeks. terminated at 4, 8, 12, 16, 20, 24 weeks after the beginning 12-week exposure period. Retention total 14C was used as an indicator reactive metabolites. isolated from analyzed using a 32P-postlabeling assay. Inhalation BaPCB resulted 100-fold higher levels end than did BaP. The halftime decline 34 ± 3 (mean SE) 6 2 At exposure, ranged 2–15 per 109 bases = 7, n 4) 10–12 11, 4); sham-exposed 0–2 1, 4). halftimes 5 One found both tentatively identified diol epoxide deoxyguanosine (BPDE) adduct. Levels BPDE significantly (p < 0.05) compared rats. There no significant differences BaPCB, although pattern different between two modes. These data point importance determining specific form potential genotoxic effects opposed relying 14C.

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