Drosophila TDP-43 RNA-Binding Protein Facilitates Association of Sister Chromatid Cohesion Proteins with Genes, Enhancers and Polycomb Response Elements.

摘要: The cohesin protein complex mediates sister chromatid cohesion and participates in transcriptional control of genes that regulate growth development. Substantial reduction activity alters transcription many without disrupting chromosome segregation. Drosophila Nipped-B loads onto chromosomes, together occupy essentially all active enhancers a large fraction genes. It is unknown why some bind high levels do not. Here we show the TBPH Lark RNA-binding proteins influence association with gene regulatory sequences. In vitro, specifically RNAs produced by occupied cohesin. By genomic chromatin immunoprecipitation these also to chromosomes at cohesin-binding genes, enhancers, Polycomb response elements (PREs). RNAi depletion reveals facilitates reduces binding promoters aids their several enhancers. Conversely, sequences, interacts affinity chromatography experiments. Blocking does not ablate indicating required maintain once established. These findings demonstrate help govern