Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.).
作者： Qing A. Yao , Ecaterina Simion , Manilal William , Joan Krochko , Ken J. Kasha
DOI: 10.1139/G97-075
关键词:
摘要: Transgenic barley plants were produced by the direct delivery of plasmid DNA into isolated microspores cv. Igri using high velocity microprojectiles. The pAHC25 contained uidA and bar genes, each under control a maize Ubi1 promoter. Bombarded cultured selected on solid medium containing varying concentrations (2–5 mg/L) Basta herbicide active agent bialaphos. effectiveness selection with bialaphos depended its interaction component glutamine. Six transgenic (R0) obtained, presence genes their integration nuclear in transformed R0 confirmed PCR Southern blot analysis. Phosphinothricin acetyltransferase activity was observed all six plants, whereas none showed β-glucuronidase (GUS) histochemical GUS assays. Two haploid sterile; one them trisomic partially remainder dip...
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