Self-Assembled Polyelectrolyte Nanoparticles as Fluorophore-Free Contrast Agents for Multicolor Optical Imaging

摘要: In this work, we describe the fabrication of self-assembled polyelectrolyte nanoparticles that provide a multicolor optical imaging modality. Poly(γ-glutamic acid)(γ-PGA) formed through electrostatic interactions with two different cationic polymers: poly(L-lysine)(PLL) and chitosan. The γ-PGA/PLL γ-PGA/chitosan were crosslinked by glutaraldehyde. Crosslinking ionic glutaraldehyde not only stabilized but also generated strong autofluorescence signal. Fluorescent Schiff base bonds (C=N) double (C=C) simultaneously crosslinking amine moiety polyelectrolytes monomeric or polymeric unique properties resulted from analyzed using UV/Vis fluorescence spectroscopy. We observed intensity could be regulated adjusting crosslinker concentration reaction time. exhibited high performance in labeling monitoring therapeutic immune cells (macrophages dendritic cells). These are expected to promising contrast agent for labeling, detection, cells.