Protease-catalyzed synthetic reactions and immobilization-activation of the enzymes in hydrophilic organic solvents.

摘要: Abstract The catalytic feature of serine proteases for synthetic reactions in hydrophilic organic solvents and effects immobilization by complexation with polysaccharides are described. Free α-chymotrypsin subtilisin Carlsberg catalyze esterification, transesterification, peptide synthesis hydro-organic cosolvents less than 10% water. Subtilisin BPN′ is catalytically active. medium on the reaction kinetics product yield were investigated terms nature solvent water content systems. substrate- stereo-specificities enzymes suggest that maintain their native conformations these low-water solvents. activities markedly increase or polysaccharides, such as chitin chitosan. results rate measurements primary role support materials activation substrate concentration at sites.