Cloning and Expression of a P2yPurinoceptor from the Adult Bovine Corpus Callosum

摘要: Abstract We have isolated an ATP receptor clone by screening a bovine corpus callosum cDNA library. The includes one open reading frame encoding for protein of 373 amino acid residues (42 kDa) which belongs to the G-protein-coupled superfamily. In Xenopus oocytes, this expressed that triggered oscillatory current in response (EC 50  ≈ 20 μM). This may resulted from activation phospholipase C, formation inositol trisphosphate, and release Ca 2+ , then opens Cl − channels. order potency agonists was 2-MeSATP ≈ ⪢ α,β-MeATP > adenosine, UTP ineffective, pharmacological profile consistent with P 2y purinoceptor. Northern blot analysis mRNAs various brain tissues showed gene is cerebellum, medulla, callosum, hippocampus, superior colliculus, frontal cortex, retina. situ RT-PCR transcripts many glial cells endothelial callosum. cloned play important role neuron–glial signaling under normal pathological conditions.