Quantitative kinetics footprinting of protein-DNA association reactions
作者： Mark Hsieh , Michael Brenowitz
DOI: 10.1016/S0076-6879(96)74038-7
关键词:
摘要: Publisher Summary This chapter presents implementation of the quantitative "footprinting" protocols with a commercially available quench-flow apparatus. The kinetics are based on development individual-site binding methods. technique has been conducted member Lac and Gal repressor family bacterial regulatory proteins, Escherichia coli (GalR). thermodynamics DNA by this proteins to specific sequences have extensively characterized. DNase I "footprint" titration developed implement analysis. volumes contributed reaction mixtures from sample loops syringes determined measuring dilution dye that is sequentially loaded into individual components time nuclease exposure protein-DNA-containing solutions physical parameters flow system. rate liquid linear velocity motor cross-sectional area drive syringe.
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sci-hub.se 参考文章(25)
Kenneth S. Koblan, David L. Bain, Dorothy Beckett, Madeline A. Shea, Gary K. Ackers, Analysis of site-specific interaction parameters in protein-DNA complexes. Methods in Enzymology. ,vol. 210, pp. 405- 425 ,(1992) , 10.1016/0076-6879(92)10021-5
R.B. O'Gorman, M. Dunaway, K.S. Matthews, DNA binding characteristics of lactose repressor and the trypsin-resistant core repressor. Journal of Biological Chemistry. ,vol. 255, pp. 10100- 10106 ,(1980) , 10.1016/S0021-9258(19)70433-5
Michael Brenowitz, Donald F. Senear, Madeline A. Shea, Gary K. Ackers, [9] Quantitative DNase footprint titration: A method for studying protein-DNA interactions Methods in Enzymology. ,vol. 130, pp. 132- 181 ,(1986) , 10.1016/0076-6879(86)30011-9
Gary K. Ackers, Madeline A. Shea, Francine R. Smith, Free energy coupling within macromolecules Journal of Molecular Biology. ,vol. 170, pp. 223- 242 ,(1983) , 10.1016/S0022-2836(83)80234-4
R.B. O'Gorman, J.M. Rosenberg, O.B. Kallai, R.E. Dickerson, K. Itakura, A.D. Riggs, K.S. Matthews, Equilibrium binding of inducer to lac repressor.operator DNA complex. Journal of Biological Chemistry. ,vol. 255, pp. 10107- 10114 ,(1980) , 10.1016/S0021-9258(19)70434-7
Arthur D. Riggs, Hiromi Suzuki, Suzanne Bourgeois, Lac repressor-operator interaction. I. Equilibrium studies. Journal of Molecular Biology. ,vol. 48, pp. 67- 83 ,(1970) , 10.1016/0022-2836(70)90219-6
Michael Brenowitz, Elizabeth Jamison, Modulation of the stability of a Lac repressor mediated looped complex by temperature and ions: Allosteric regulation by chloride Biochemistry. ,vol. 32, pp. 8693- 8701 ,(1993) , 10.1021/BI00084A041
Jeung-Hoi Ha, Ruth S. Spolar, M.Thomas Record, Role of the hydrophobic effect in stability of site-specific protein-DNA complexes Journal of Molecular Biology. ,vol. 209, pp. 801- 816 ,(1989) , 10.1016/0022-2836(89)90608-6
Otto G. Berg, Robert B. Winter, Peter H. Von Hippel, Diffusion-Driven Mechanisms of Protein Translocation on Nucleic Acids. 1. Models and Theory? Biochemistry. ,vol. 20, pp. 6929- 6948 ,(1981) , 10.1021/BI00527A028
Jörg Langowski, Claus Urbanke, Ekkehard Schuppe, Construction of a microprocessor-controlled pulsed quench-flow apparatus for the study of fast chemical and biochemical reactions Analytical Biochemistry. ,vol. 142, pp. 91- 97 ,(1984) , 10.1016/0003-2697(84)90521-9