The Transition between Transcriptional Initiation and Elongation in E. coli Is Highly Variable and Often Rate Limiting
作者： Nikos B. Reppas , Joseph T. Wade , George M. Church , Kevin Struhl
DOI: 10.1016/J.MOLCEL.2006.10.030
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摘要: … genomic DNA was then incubated with Klenow DNA polymerase, 10 μg random hexamer, and dNTP mix containing aminoallyl-dUTP for 4 hr at 37C. Amplified genomic DNA was then …
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