Purification and properties of flagellar outer doublet tubulin from Naegleria gruberi and a radioimmune assay for tubulin.
作者： Joel D. Kowit , Chandler Fulton
DOI: 10.1016/S0021-9258(19)42621-5
关键词:
摘要: Outer doublet tubulin has been purified from flagella of differentiated cells the amebo-flagellate Naegleria gruberi. The flagellar is similar to other tubulins in molecular weight (55,000), amino acid composition, electrophoretic mobility, and nucleotide composition. However, protein contains 2 moles guanine per 55,000 g protein, twice that usually found tubulin. A rabbit antiserum against was shown contain antibodies specific for Purified iodinated with 125I used a radioimmune assay measure levels antigen cells. measurements indicate increases 35- 55-fold during differentiation amebae flagellates.
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