Application of HPLC to the Separation of Lipids

摘要: It is comparatively easy to write about “separations” of lipids because, in the field lipid HPLC, separation techniques as such have made far more progress than detector developments. Today we can separate most mixtures that want separate, but all too often cannot detect separated peaks, or at least quantitate them. must be kept mind, therefore, a lot published work nowadays modes used were chosen primarily with an eye on available — and not because particular mode best one molecules groups wants separate. Thus, gradient would RI detector; benzene toluene for silver nitrate column needs detection via short-wave UV, “group” quantitation there no equal sensitivity saturated unsaturated present together peak.