Method for detecting reactive metabolites using a combination of glutathione and a stable isotopic derivative of glutathione and mass spectroscopy-based pattern recognition
作者： Zhengyin Yan , Gary W. Caldwell
DOI:
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摘要: The present invention is directed to a method for detecting reactive metabolites using combination of glutathione and stable isotopic derivative mass spectroscopy. More specifically, the selectively detects by eliminating false positives arising from non-reactive components including both unreactive reaction mixture.
lens.org 参考文章(15)
STU BORMAN, NIPPING BAD DRUGS IN THE BUD Chemical & Engineering News. ,vol. 82, pp. 36- 39 ,(2004) , 10.1021/CEN-V082N020.P036
Timothy Harper, William Humphreys, Jinping Gan, Fluorescently labeled thiol-containing trapping agent for the quantitation and identification of reactive metabolites in vitro ,(2005)
Fred E. Regnier, Roujian Zhang, Materials and methods for controlling isotope effects during fractionation of analytes ,(2002)
Nicolas Andre Stirling Stewart, Harvey Kaplan, Increased sensitivity of peptide detection with matrix-assisted laser desorption/ionization mass spectrometry by in vacuo methylation of amino groups United States Patent and Trademark Office. ,(2002)
Hassan G. Fouda, Michael J. Avery, Weichao G. Chen, High throughput screen reducing drug candidate attrition ,(2001)
A E P Adang, J Brussee, A van der Gen, G J Mulder, The glutathione-binding site in glutathione S-transferases. Investigation of the cysteinyl, glycyl and γ-glutamyl domains Biochemical Journal. ,vol. 269, pp. 47- 54 ,(1990) , 10.1042/BJ2690047
Karel Domansky, Steven R. Tannenbaum, Linda G. Griffith, Charles D. Thompson, Mark J. Powers, Vascularized perfused microtissue/micro-organ arrays ,(1999)
Paul G. Pearson, William N. Howald, Sidney D. Nelson, Screening strategy for the detection of derivatized glutathione conjugates by tandem mass spectrometry Analytical Chemistry. ,vol. 62, pp. 1827- 1836 ,(1990) , 10.1021/AC00216A019
William J. A. VandenHeuvel, Drug Metabolite Identification: Stable Isotope Methods The Journal of Clinical Pharmacology. ,vol. 26, pp. 427- 434 ,(1986) , 10.1002/J.1552-4604.1986.TB03553.X
Jinping Gan, Timothy W. Harper, Mei-Mann Hsueh, Qinling Qu, W. Griffith Humphreys, Dansyl Glutathione as a Trapping Agent for the Quantitative Estimation and Identification of Reactive Metabolites Chemical Research in Toxicology. ,vol. 18, pp. 896- 903 ,(2005) , 10.1021/TX0496791