Efficient construction of a recombinant adenovirus vector by an improved in vitro ligation method
作者： Hiroyuki Mizuguchi , Mark A. Kay
DOI: 10.1089/HUM.1998.9.17-2577
关键词:
摘要: ABSTRACT An efficient method for constructing a recombinant adenovirus (Ad) vector, based on an in vitro ligation, has been developed. To insert the foreign gene into adenoviral DNA, we introduced three unique restriction sites, I-CeuI, SwaI, and PI-SceI, E1 deletion site of vector plasmid, which contains complete E1, E3-deleted type 5 genome. I-CeuI PI-SceI are intron-encoded endonucleases with sequence specificity at least 9–10 11 bp, respectively. A shuttle pHM3, containing multiple cloning sites between was constructed. After interest inserted this plasmid E1-deleted could be easily prepared by ligation using sites. SwaI digestion products prevented production parental genome (null vector). transformation E. coli, more than 90% transformants had corr...
stanford.edu
liebertonline.com
researchgate.net 参考文章(20)
A Lieber, C Y He, I Kirillova, M A Kay, Recombinant adenoviruses with large deletions generated by Cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. Journal of Virology. ,vol. 70, pp. 8944- 8960 ,(1996) , 10.1128/JVI.70.12.8944-8960.1996
M. Rosenfeld, W Siegfried, K Yoshimura, K Yoneyama, M Fukayama, L. Stier, P. Paakko, P Gilardi, L. Stratford-Perricaudet, M Perricaudet, al. et, Adenovirus-mediated transfer of a recombinant alpha 1-antitrypsin gene to the lung epithelium in vivo Science. ,vol. 252, pp. 431- 434 ,(1991) , 10.1126/SCIENCE.2017680
Mark A. Kay, Qiutang Li, Ta-Jen Liu, Frances Leland, Carol Toman, Milton Finegold, Savio L. C. Woo, Hepatic Gene Therapy: Persistent Expression of Human α1-Antitrypsin in Mice after Direct Gene Delivery In Vivo Human Gene Therapy. ,vol. 3, pp. 641- 647 ,(1992) , 10.1089/HUM.1992.3.6-641
Karen F. Kozarsky, James M. Wilson, Gene therapy: adenovirus vectors. Current Opinion in Genetics & Development. ,vol. 3, pp. 499- 503 ,(1993) , 10.1016/0959-437X(93)90126-A
Joan E. Wilson, Paul M. Macdonald, Formation of germ cells in Drosophila Current Opinion in Genetics & Development. ,vol. 3, pp. 562- 565 ,(1993) , 10.1016/0959-437X(93)90091-3
Kathleen L. Berkner, Phillip A. Sharp, Generation of adenovirus by transfection of plasmids Nucleic Acids Research. ,vol. 11, pp. 6003- 6020 ,(1983) , 10.1093/NAR/11.17.6003
S. Miyake, M. Makimura, Y. Kanegae, S. Harada, Y. Sato, K. Takamori, C. Tokuda, I. Saito, Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 1320- 1324 ,(1996) , 10.1073/PNAS.93.3.1320
G. Ketner, F. Spencer, S. Tugendreich, C. Connelly, P. Hieter, Efficient manipulation of the human adenovirus genome as an infectious yeast artificial chromosome clone Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 6186- 6190 ,(1994) , 10.1073/PNAS.91.13.6186
Frederick S. Gimble, Jeremy Thorner, Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature. ,vol. 357, pp. 301- 306 ,(1992) , 10.1038/357301A0
Albert B. Deisseroth, Use of the cosmid adenoviral vector cloning system for the in vitro construction of recombinant adenoviral vectors Human Gene Therapy. ,vol. 8, pp. 1321- 1330 ,(1997) , 10.1089/HUM.1997.8.11-1321