Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine (TCM) matrine with Ga(III), Au(III), Sn(IV) ions, and DNA binding studies.

摘要: Three new compounds of Ga(III), Au(III), Sn(IV) with matrine (MT), [H-MT][GaCl(4)] (1), [H-MT][AuCl(4)] (2) and [Sn(H-MT)Cl(5)] (3), have been synthesized characterized by elemental analysis, IR, ESI-MS single crystal X-ray diffraction methods. The structural analyses indicate that 1 2 are ionic compounds, whereas 3 is a tin(IV) complex formed the monodentate MT via its carbonyl oxygen atom coordinating to Sn(IV). Their in vitro cytotoxicity towards eight selected tumour cell lines has evaluated MTT (3-[4,5-Dimentylthiazole-2-yl]-2,5-diphenpyltetra-zolium bromide) method, exhibit enhanced activity, such as SW480, HeLa, HepG2 MCF-7, which exceeds cisplatin, display synergistic contribution their components. cycle show 1, arrest at G(2)/M phase. Interactions these calf thymus DNA (ct-DNA) investigated spectroscopic analyses. planar extension intercalative metal-matrine increases interaction DNA, indicating cationic metal ions configuration intercalated will affect extent interaction. Compound 2, [H-MT][AuCl(4)], exhibits more intensive binding ability may correlate intercalation other action mode. circular dichroism spectra ct-DNA bound metal-MT also suggest interacted does not influence secondary structure. Furthermore, both effective inhibition topoisomerase (TOPO I) concentration 50 μM, while compound do not.