Molecular mutation profile of Pfcrt and Pfmdr1 in Plasmodium falciparum isolates from Bioko Island, Equatorial Guinea.

摘要: Abstract Background Antimalarial drug resistance is a primary public health problem. Haplotypes of pfcrt and pfmdr1 gene have been implicated to be molecular markers chloroquine (CQ) resistance. This study aims explore mutation distribution Pfcrt Pfmdr1 in Bioko Island, Equatorial Guinea (EG). Methods Blood samples were collected from different districts Bioko. The single nucleotide polymorphisms (codons 72 76) 86, 130, 184, 1034, 1042, 1109 1246) assessed by nested PCR with DNA sequencing haplotype prevalences also determined. Results Analysis mutations was successful 151 157 respectively out the 172 taken for this study. found 98.67% 89.81% isolates, respectively. 74–76, 184 92.05%, 50.32%, 87.26% mostly type, Three haplotypes coding 72–76 including CVMNK, CV IET , M/I N/E K/T which accounted 1.33%, 6.62%, No Pfmdr1-N1 codon at 130 Pfmdr1-N2 (S1034C, N1042D, V1109I, D1246Y) detected. types 86 NY, Y Y, N F YF Y/F YY/F 10.19%, 2.55%, 33.76%, 45.22%, 5.73% Conclusion High prevalence double confirm high-level CQ (CQR) might suggest reduced susceptibility Plasmodium falciparum isolates AQ Bioko, EG. It establishes fundamental data detection P. CQR will promotes surveillance level