Identification of Expressed Sequences on Human Chromosome 9q32–34

摘要: Ozelius and colleagues have recently mapped the gene responsible for neuromuscular disease idiopathic torsion dystonia (DYT1) to human chromosome 9q32–34. Our goal is identify candidate genes dystonia, as well other neurologically important localized on To accomplish this we identified transcribed sequences within a 3,000 clone collection assembled from 9q32–34-specific library. Dystonia candidates are being assessed based their expression patterns localization with respect DYT1 locus. cDNA probes various brain peripheral tissues were used screen enabling us elucidate tissue of corresponding each clone. This resulted in identification 143 9q32–34 transcripts, thirty-three which expressed brain-specific manner thus gene. Since none these transcripts was specifically putative dystonic target basal ganglia, directional tag PCR subtraction method construct probe enriched striatal sequences. Screening subtracted several additional clones preferentially striatum.