Cotesia congregata Bracovirus Circles Encoding PTP and Ankyrin Genes Integrate into the DNA of Parasitized Manduca sexta Hemocytes.

摘要: Polydnaviruses (PDVs) are essential for the parasitism success of tens thousands species parasitoid wasps. PDVs present in wasp genomes as proviruses, which serve template production double-stranded circular viral DNA carrying virulence genes that injected into lepidopteran hosts. PDV circles do not contain coding particle production, thereby impeding replication caterpillar hosts during parasitism. Here, we investigated fate Cotesia congregata bracovirus tobacco hornworm, Manduca sexta, by Sequences sharing similarities with host integration motifs (HIMs) Microplitis demolitor (MdBV) involved could be identified 12 CcBV circles, encode PTP and VANK gene families immune disruption. A PCR approach performed on a subset these indicated they persisted parasitized M. sexta hemocytes linear forms, possibly integrated DNA. Furthermore, using primer extension capture method based HIMs high-throughput sequencing, show 8 out 9 tested were hemocyte genomic had occurred specifically HIM, indicating an HIM-mediated specific mechanism was their integration. Investigation BV circle insertion sites at genome scale revealed certain regions appeared to enriched insertions, but no target site identified.IMPORTANCE The identification efficient shared several opens question its role braconid success. Indeed, results obtained here massive somatic cells each event host. Given bracoviruses replicate infected cells, sequences might allow proteins within newly dividing continue develop this process basis understand how mediate recently flux between wasps Lepidoptera frequency horizontal transfer events nature.