Expression of two variants of the human μ opioid receptor mRNA in SK-N-SH cells and human brain
作者： Lance A. Bare , Erik Mansson , Dingmming Yang
DOI: 10.1016/0014-5793(94)01129-X
关键词:
摘要: A partial μ opioid receptor gene was isolated from a human genomic library using mouse δ cDNA as probe. Using information this clone and the published receptor, MOR1 SK-N-SH mRNA that codes for variant of mRNA, MOR1A. The presence MOR1A is also shown in brain RT-PCR. differs 3′ terminal intron has not been removed. An in-frame termination codon found four amino acids after 5′ consensus splice site, making eight shorter than MOR1. Both receptors show similar ligand binding coupling to cAMP CHO-K1 cells. C-terminal differences between could have effects on or transport localization.
elsevier.com
sci-hub.se 参考文章(21)
Jia-Bei Wang, Peter S. Johnson, Antonio M. Persico, Anita L. Hawkins, Constance A. Griffin, George R. Uhl, Human μ opiate receptor: cDNA and genomic clones, pharmacologic characterization and chromosomal assignment FEBS Letters. ,vol. 338, pp. 217- 222 ,(1994) , 10.1016/0014-5793(94)80368-4
T. Reisine, K. Raynor, G. I. Bell, Hayeoung Kong, K. Yasuda, Yan Chen, Lei Yu, Pharmacological characterization of the cloned kappa-, delta-, and mu-opioid receptors. Molecular Pharmacology. ,vol. 45, pp. 330- 334 ,(1994)
Philip S Portoghese, M. Sofuoglu, A. E. Takemori, Differential antagonism of delta opioid agonists by naltrindole and its benzofuran analog (NTB) in mice: evidence for delta opioid receptor subtypes. Journal of Pharmacology and Experimental Therapeutics. ,vol. 257, pp. 676- 680 ,(1991)
Gavril W. Pasternak, Pharmacological mechanisms of opioid analgesics. Clinical Neuropharmacology. ,vol. 16, pp. 1- 18 ,(1993) , 10.1097/00002826-199302000-00001
Donald D. Koblin, Joan E. Deady, Sensitivity to alcohol in mice with an altered brain fatty acid composition Life Sciences. ,vol. 28, pp. 1889- 1896 ,(1981) , 10.1016/0024-3205(81)90295-2
Miyuki Nishi, Hiroshi Takeshima, Kazuhiko Fukuda, Shigehisa Kato, Kenjiro Mori, cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for κ-subtype-selective ligands FEBS Letters. ,vol. 330, pp. 77- 80 ,(1993) , 10.1016/0014-5793(93)80923-I
Mirko Vanetti, Monika Kouba, Xiaomin Wang, Gudrun Vogt, Volker Höllt, Cloning and expression of a novel mouse somatostatin receptor (SSTR2B) FEBS Letters. ,vol. 311, pp. 290- 294 ,(1992) , 10.1016/0014-5793(92)81122-3
Peter J Munson, David Rodbard, None, Ligand: a versatile computerized approach for characterization of ligand-binding systems. Analytical Biochemistry. ,vol. 107, pp. 220- 239 ,(1980) , 10.1016/0003-2697(80)90515-1
C. Evans, D. Keith, H Morrison, K Magendzo, R. Edwards, Cloning of a delta opioid receptor by functional expression Science. ,vol. 258, pp. 1952- 1955 ,(1992) , 10.1126/SCIENCE.1335167
F. Meng, G. X. Xie, R. C. Thompson, A. Mansour, A. Goldstein, S. J. Watson, H. Akil, Cloning and pharmacological characterization of a rat kappa opioid receptor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 9954- 9958 ,(1993) , 10.1073/PNAS.90.21.9954