Amifostine Protects Against Chemotherapy-induced Neurotoxicity: An In Vitro Investigation

摘要: Background Peripheral neurotoxicity is a dose-limiting side-effect of number effective chemotherapeutic agents. Neuroprotective agents may help to reduce neurotoxicity, thus allowing the intensification cytostatic therapy in patients. Materials and methods In this vitro study, using rat pheochromocytoma cell line PC-12 neurite-outgrowth assay, potential amifostine protect against cisplatin-, paclitaxel- vincristine-induced was investigated Amifostine described as selectively protecting normal tissue not tumour tissue. The effect on kill XTT colony forming assay. Results Paclitaxel vincristine both caused significant reduction percentage cells expressing neurites. Co-incubation with significantly increased neurites paclitaxel-induced but neurotoxicity. Post-incubation also proved partly reverse already existing cisplatin-induced paclitaxel-, or did cisplatin- cytotoxicity, However, stimulation clonogenic capacity observed when coincubated cisplatin. Conclusion protects model. Furthermore, has role proliferative needs further investigation.