Bacterial biofilm formation is variably inhibited by different formulations of antibiotic-loaded bone cement in vitro.

摘要: The aim of the present study was to quantitatively assess biofilm growth on surface bone cements discs containing different antibiotics, including colistin and linezolid. Biofilms methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, epidermidis were grown cement for 96 h. Biofilm amounts measured by confocal laser microscopy using live/dead staining dedicated software at time intervals (48, 72, 96 h). Bone vancomycin not effective reducing MRSA formation 96 h following bacterial inoculation. At a comparable interval, linezolid-, clindamycin-, aminoglycoside-loaded still active against this biofilm. 72- 96-h observations, S. only tobramycin gentamicin discs. P. aeruginosa biofilms loaded with all starting from 48-h observation, whereas no detected or antibiotics have variable time-dependent windows activity in inhibiting formation. effectiveness is questionable. clinically relevant, because it suggests that adding right antibiotic could be promising approach treat periprosthetic infections. Indeed, antibiofilm antibiotic-loaded preoperatively assessed current methodology two-stage exchange procedures.