Comparison of two different PCR detection methods. Application to the diagnosis of pulmonary tuberculosis.

摘要: The objectives are to assess the influence of detection amplified DNA fragment on sensitivity and specificity polymerase chain reaction (PCR). One hundred seventy-five sputum samples from 123 patients were processed. Sixty taken 60 subjects without tuberculosis, rest with tuberculosis confirmed by culture. A IS6110 sequence Mycobacterium which was detected using two different methods, amplified. methods used a digoxigenin-labeled specific probe chemiluminescent development reamplification (nested PCR) combined agarose gel electrophoresis. Sensitivity 75.65% 100%. Using nested PCR technique, rose 93.04%, but decreased 96.6%. is quick adequate way diagnose pulmonary in cases where staining negative yet there clinical suspicion even though standardization process large scale evaluation still needed determine its true usefulness.