Detecting the ability of viral, bacterial and eukaryotic replication proteins to track along DNA.

摘要: The phage T4 gene 45 protein (gp45), Escherichia coli beta and the eukaryotic proliferating cell nuclear antigen (PCNA) function in replication as processivity factors of their corresponding DNA polymerases. gp45 also functions transcriptional activator that connects expression viral late genes to replication. tracking is an essential component transcription regulatory gp45. ability gp45, PCNA track along has been analyzed by photocrosslinking. Each these proteins must be loaded onto a species-specific assembly factor. For beta, density traffic determined dynamic balance between continuous loading unloading, dependent on interaction with conjugate single-stranded binding protein.