Anti-proliferative effect of IFN-gamma in immune regulation. I. IFN-gamma inhibits the proliferation of Th2 but not Th1 murine helper T lymphocyte clones.

摘要: A biphasic dose-response curve was observed when the IL-1-dependent HTL clone D10 exposed to IL-1 plus supernatants from some activated T cell clones but not others. The active component that inhibited proliferation at high concentrations of these appeared be IFN-gamma based on following findings: 1) pattern responsiveness correlated with presence in supernatants; 2) an anti-IFN-gamma mAb augmented cells 3) rIFN-gamma profoundly response stimulated rIL-1 supernatant or rIL-4; 4) rIL-2 also by rIFN-gamma, although a lesser extent. additional Th2 rIL-4 implicating as inhibitory lymphokine for general. did affect two Th1 clones, nor it unconventional which produces both IL-4 and proliferates IL-2 IL-1-independent fashion. Ag immobilized anti-CD3 antibody blocked whereas production stimuli unaffected, indicating general function specifically inhibited. Collectively, data implicate suppressive factor subset designated Th2, suggest relative amounts various lymphokines present during immune may direct types increase number.