Anti-inflammatory Effects of Fungal Metabolites in Mouse Intestine as Revealed by In vitro Models.

摘要: Inflammatory bowel diseases (IBD), which include Crohn´s disease and ulcerative colitis, are chronic inflammatory disorders that can affect the whole gastrointestinal tract or colonic mucosal layer. Current therapies aiming to suppress exaggerated immune response in IBD largely rely on compounds with non-satisfying effects side-effects. Therefore, new therapeutical options needed. In present study, we investigated anti-inflammatory of fungal metabolites, galiellalactone dehydrocurvularin both an vitro intestinal inflammation model, as well isolated myenteric plexus enterocyte cells. Administration a pro-inflammatory cytokine mix through mesenteric artery segments caused up-regulation marker genes. Treatment murine by application significantly prevented expression genes mRNA protein level. Comparable results perfused intestine treatment primary enteric nervous system (ENS) cells from reduced cytokines such IL-6, TNF-α, IL-1β enzymes COX-2 iNOS levels. Similar metabolites were observed human colorectal adenocarcinoma cell line DLD-1 after stimulation IFN-γ (10 ng/ml), TNF-α ng/ml) (5 ng/ml). Our show mesenterially model provides reliable tool for screening therapeutics limited amounts test compounds. Furthermore could characterize two novel active compounds, interesting candidates studies animal models IBD.