Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors

摘要: B lymphocyte production in murine bone marrow is negatively regulated by sex steroids and the aim of this study was to identify early hormone sensitive checkpoints. Estrogen (E2) treatment reduced cμ + pre-B cells, a change that occurred concomitantly with decreased Ig gene rearrangements rag-1 transcripts. lineage precursors transgenic mice, demonstrating hormonal regulation independent recombination process. Bcl-2 mice were resistant estrogen treatment, suggesting life/death decisions are involved regulation. A previously uncharacterized population CD43 − identified normal, transgenic, RAG −/− after revealing down-regulation can occur heavy chain expression. These cells expressed transcripts for both tdt bcl-2 , characteristics B-cell precursors. BrdU incorporation analysis revealed mitotic activity B-lineage hormone-treated mice. We conclude modulate influencing differentiation, proliferation, survival findings informative about mechanisms regulation, as well significance some differentiation-related events.