Incorporation of 3H-Hypoxanthine in PHA Stimulated HG-PRT Deficient Lymphocytes
作者： C. H. M. M. de Bruyn , T. L. Oei
DOI: 10.1007/978-1-4684-3294-7_28
关键词:
摘要: Autoradiography with labeled substrates on cultured fibroblasts has been used to visualize HG-PRT deficiency: cells of deficient individuals lack the ability incorporate hypoxanthine or guanine into nucleic acids, because these purine bases can not be converted their corresponding mononucleotides (1,2). The alternative pathway form IMP GMP via inosine guanosine is likely, for, although nucleoside phosphorylase present, there no definite evidence for existence inosine- kinase in human (3,4).
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