Growth and endocrine effect of growth hormone transgene dosage in diploid and triploid coho salmon.

摘要: Growth-hormone transgene dosage, polyploidy, and parental effects on growth endocrine responses have been assessed in coho salmon. Diploid fry with one or two doses grew equally, whereas later-stage juvenile homozygotes faster than hemizygotes. In contrast, hemizygotes equally after smoltification, both sea water fresh water. Triploid transgenic salmon showed impaired which could not be fully overcome additional copies. Levels of muscle GH mRNA were elevated vs. dose diploids, but triploids, a dosage effect was observed for animals carrying three doses. IGF-I levels non-transgenic animals, observed. Diploids triploids transgenes had higher plasma one-dose three-dose no further elevation. Circulating also among any triploids. The present study reveals complex interactions maternal effects, developmental stage, ploidy parameters Specifically, do always express nor that are directly correlated the number transgenes. Further, reduced rate seen triploid by increasing dosage. findings relevance understanding physiology, function, environmental risk assessments require phenotypes hemizygous homozygous populations.