Pathogenic and Opportunistic Free-Living Amebae

摘要: Small, free-living amebae belonging to the genera Naegleria, Acanthamoeba, and Balamuthia have been identified as agents of central nervous system (CNS) infections humans other animals. The concept that these small, may occur human pathogens was proposed by Culbertson colleagues, who isolated Acanthamoeba sp. genus contains many 24 species in three groups, with groupings based largely on morphologic characteristics. chapter talks about clinical significance Naegleria Meningoencephalitis, Encephalitis, (Leptomyxid) Keratitis. It outlines recommended procedure for isolating pathogenic from biological specimens. Identification living organisms level is characteristic patterns locomotion, features trophozoite cyst forms, results enflagellation experiments. spp can easily be cultivated axenically, without addition serum or host tissue, different types nutrient media, e.g., proteose peptone-yeast extract-glucose medium, Trypticase soy broth chemically defined medium. serologic techniques discussed this developed research tools are not routinely available laboratories. Most laboratories rely agar plate technique isolation identification free-living, amebae, techniques, like PCR, sometimes even feasible. usually send specimens an outside laboratory Centers Disease Control (CDC) interpretation.