Serological Investigation of Laboratory-Confirmed and Suspected Ebola Virus Disease Patients During the Late Phase of the Ebola Outbreak in Sierra Leone.
作者： Yang Liu , YuLan Sun , Wei Wu , AQian Li , XianDa Yang
DOI: 10.1007/S12250-018-0044-Z
关键词:
摘要: This study aimed to investigate the serological characteristics of Ebola virus (EBOV) infection during late phase outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected disease (EVD) cases assessed March December 2015, were analyzed via real-time reverse transcription polymerase chain reaction (RT-PCR) for viral RNA and enzyme-linked immunosorbent assay (ELISA) Luminex detect antibodies against EBOV. Viral load EBOV-specific IgM/IgG titers displayed a declining trend 2015. decreased rapidly at earlier stages after onset, while IgM IgG still persisted 58.1% (18/31) 93.5% (29/31) confirmed EVD patients 3.8% (25/663) 17.8% (118/663) RNA-negative later phase, respectively. Dynamic analysis longitudinally collected eight revealed typically reversed trends increasing and/or response EBOV infection. The present results indicate that certain populations Leone developed immunity an outbreak, providing novel insights into risk assessment infections among human populations.
virosin.org
springer.com
sci-hub.se 参考文章(31)
Jay B. Varkey, Jessica G. Shantha, Ian Crozier, Colleen S. Kraft, G. Marshall Lyon, Aneesh K. Mehta, Gokul Kumar, Justine R. Smith, Markus H. Kainulainen, Shannon Whitmer, Ute Ströher, Timothy M. Uyeki, Bruce S. Ribner, Steven Yeh, Persistence of Ebola Virus in Ocular Fluid during Convalescence The New England Journal of Medicine. ,vol. 372, pp. 2423- 2427 ,(2015) , 10.1056/NEJMOA1500306
Timothy R. Southern, Lori D. Racsa, César G. Albariño, Paul D. Fey, Steven H. Hinrichs, Caitlin N. Murphy, Vicki L. Herrera, Anthony R. Sambol, Charles E. Hill, Emily L. Ryan, Colleen S. Kraft, Shelley Campbell, Tara K. Sealy, Amy Schuh, James C. Ritchie, G. Marshall Lyon, Aneesh K. Mehta, Jay B. Varkey, Bruce S. Ribner, Kent P. Brantly, Ute Ströher, Peter C. Iwen, Eileen M. Burd, Comparison of FilmArray and Quantitative Real-Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens Journal of Clinical Microbiology. ,vol. 53, pp. 2956- 2960 ,(2015) , 10.1128/JCM.01317-15
Hui-Jun Lu, Jun Qian, David Kargbo, Xiao-Guang Zhang, Fan Yang, Yi Hu, Yang Sun, Yu-Xi Cao, Yong-Qiang Deng, Hao-Xiang Su, Foday Dafae, Yu Sun, Cheng-Yu Wang, Wei-Min Nie, Chang-Qing Bai, Zhi-Ping Xia, Kun Liu, Brima Kargbo, George F Gao, Jia-Fu Jiang, None, Ebola Virus Outbreak Investigation, Sierra Leone, September 28–November 11, 2014 Emerging Infectious Diseases. ,vol. 21, pp. 1921- 1927 ,(2015) , 10.3201/EID2111.150582
Andreas Lucht, Roland Grunow, Peggy Möller, Heinz Feldmann, Stephan Becker, Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus Journal of Virological Methods. ,vol. 111, pp. 21- 28 ,(2003) , 10.1016/S0166-0934(03)00131-9
Zheng Pang, Aqian Li, Jiandong Li, Jing Qu, Chengcheng He, Shuo Zhang, Chuan Li, Quanfu Zhang, Mifang Liang, Dexin Li, Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses PLoS ONE. ,vol. 9, pp. e95635- ,(2014) , 10.1371/JOURNAL.PONE.0095635
V. Rougeron, H. Feldmann, G. Grard, S. Becker, E.M. Leroy, Ebola and Marburg haemorrhagic fever. Journal of Clinical Virology. ,vol. 64, pp. 111- 119 ,(2015) , 10.1016/J.JCV.2015.01.014
Randal J. Schoepp, Cynthia A. Rossi, Sheik H. Khan, Augustine Goba, Joseph N. Fair, Undiagnosed Acute Viral Febrile Illnesses, Sierra Leone Emerging Infectious Diseases. ,vol. 20, pp. 1176- 1182 ,(2014) , 10.3201/EID2007.131265
G. F. Gao, Y. Feng, On the ground in Sierra Leone Science. ,vol. 346, pp. 666- 666 ,(2014) , 10.1126/SCIENCE.346.6209.666
Wei Wu, Shuo Zhang, Jing Qu, Quanfu Zhang, Chuan Li, Jiandong Li, Cong Jin, Mifang Liang, Dexin Li, Simultaneous detection of IgG antibodies associated with viral hemorrhagic fever by a multiplexed Luminex-based immunoassay Virus Research. ,vol. 187, pp. 84- 90 ,(2014) , 10.1016/J.VIRUSRES.2013.12.037
Sylvain Baize, Delphine Pannetier, Lisa Oestereich, Toni Rieger, Lamine Koivogui, N'Faly Magassouba, Barrè Soropogui, Mamadou Saliou Sow, Sakoba Keïta, Hilde De Clerck, Amanda Tiffany, Gemma Dominguez, Mathieu Loua, Alexis Traoré, Moussa Kolié, Emmanuel Roland Malano, Emmanuel Heleze, Anne Bocquin, Stephane Mély, Hervé Raoul, Valérie Caro, Dániel Cadar, Martin Gabriel, Meike Pahlmann, Dennis Tappe, Jonas Schmidt-Chanasit, Benido Impouma, Abdoul Karim Diallo, Pierre Formenty, Michel Van Herp, Stephan Günther, Emergence of Zaire Ebola Virus Disease in Guinea The New England Journal of Medicine. ,vol. 371, pp. 1418- 1425 ,(2014) , 10.1056/NEJMOA1404505