Use of monoclonal antibodies to detect Mn(II)-peroxidase in birch wood degraded by Phanerochaete chrysosporium

摘要: A monoclonal antibody (Mab) produced to purified Mn(II)-peroxidase was visualized on and within cell corners of birch wood degraded by Phanerochaete chrysosporium using colloidal gold immuno-transmission electron microscopy techniques. Labelling the fungal membrane wall also observed. The same Mab used visualize penetration extracellular metabolite extracts, infiltrated into previously decayed wood. Binding antibodies lignin-rich corner region middle lamella in P. observed sectioned blocks with crude extracts from chrysospirium liquid cultures. When a control antiserum, metabolites Postia (Poria) placenta cross-reactive cellulase, labelling, cellulose rich walls were labelled. only noted what has been described as nonor poorly lignified regions.