Acetylcholinesterase biosensor based on chitosan/prussian blue/multiwall carbon nanotubes/hollow gold nanospheres nanocomposite film by one-stepelectrodeposition
作者： Chen Zhai , Xia Sun , Wenping Zhao , Zhili Gong , Xiangyou Wang
DOI: 10.1016/J.BIOS.2012.10.058
关键词:
摘要: In this paper, chitosan–prussian blue–multiwall carbon nanotubes-hollow gold nanospheres (Chit–PB–MWNTs–HGNs) film was fabricated onto the electrode surface by one-step electrodeposition method; and then acetylcholinesterase (AChE) Nafion were modified to prepare an AChE biosensor. Incorporating MWNTs HGNs into Chit–PB hybrid promoted electron transfer reaction, enhanced electrochemical response improved microarchitecture of surface. The morphologies electrochemistry properties composite investigated using scanning microscopy, transmission cyclic voltammetry impedance spectroscopy, respectively. Parameters affecting biosensor such as pH, enzyme loading inhibition time optimized. Based on pesticides activity, malathion, chlorpyrifos, monocrotophos carbofuran model compounds, showed a wide range, low detection limit, good reproducibility high stability. Moreover, AChE/Chit–PB–MWNTs–HGNs/Au can also be used for direct analysis practical samples, which would new promising tool pesticide analysis.
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