Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited
作者： Florence Appaix , Marie-Nathalie Minatchy , Catherine Riva-Lavieille , Jose Olivares , Bruno Antonsson
DOI: 10.1016/S0005-2728(00)00098-0
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摘要: This paper recalls the earlier work by Keilin, Margoliash and others at beginning of 20th century shows how their results can be used for rapid solution new problems modern science. It describes a simple spectrophotometric method quantitative determination cytochrome c release from isolated mitochondria or permeabilized cells induced proapoptotic proteins. For this, Soret (gamma) peak 414 nm in spectrum is used. The assay are accord with those oxygraphic c-dependent respiration cardiomyocytes.
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