Hypoxia-Inducible Factor α and Hif-prolyl Hydroxylase Characterization and Gene Expression in Short-Time Air-Exposed Mytilus galloprovincialis.

摘要: Aquatic organisms experience environmental hypoxia as a result of eutrophication and naturally occurring tidal cycles. Mytilus galloprovincialis, being an anoxic/hypoxic-tolerant bivalve, provides excellent model to investigate the molecular mechanisms regulating oxygen sensing. Across animal kingdom, inadequacy in supply is signalled predominantly by hypoxia-inducible factors (HIF) Hif-prolyl hydroxylases (PHD). In this study, hif-α 5'-end partial phd mRNA sequences from M. galloprovincialis were obtained. Phylogenetic characterization both HIF-α PHD putative proteins showed shared key features with respective orthologues animals strongly suggesting their crucial involvement highly conserved sensing pathway. Both transcripts displayed tissue-specific distribution prominent expression gills. Quantitative gene analysis mRNAs gills demonstrated that these sensors are transcriptionally modulated availability during short-time air exposure subsequent re-oxygenation treatments proving they critical players oxygen-sensing mussels. Remarkably, prompt transient response precocious implication transcription factor early phase adaptive Mytilus. time-dependent manner trends comparable patterns, thus central role transcriptional regulation tolerance strategies marine bivalves. These results provide information about effects deficiency identify hypoxia-responsive biomarker genes mussels applicable ecotoxicological studies natural areas.