A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance

作者： Yee-yung Charng , Wen-tzu Chi , Hsiang-chin Liu , Shih-hsun Chang , Chun-neng Wang

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摘要: The expression of heat shock proteins (Hsps) induced by nonlethal treatment confers acquired thermotolerance (AT) toorganisms against subsequent challenges otherwise lethal temperature. After the stress signal is removed, AT graduallydecays, with decreased Hsps during recovery. ATof sufﬁcient duration critical for sessile organisms such as plants to surviverepeated in their environment, but little known regarding its regulation. To identify potential regulatorycomponents, we took a reverse genetics approach screening Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutantsthat show after long recovery (2 d) under nonstress conditions following an acclimation heattreatment. Among tested mutants corresponding 48 heat-induced genes, only transcription factor HsfA2knockout mutant showed obvious phenotype. Following pretreatment at 37 C, line was more sensitive severeheat than wild type not short periods, and this could be complemented introduction ofa wild-type copy HsfA2 gene. Quantitative hypocotyl elongation assay also revealed that decayed faster theabsence HsfA2. Signiﬁcant reduction transcript levels several highly heat-inducible genes observed 4 h or 2 prolonged stress. Immunoblot analysis Hsa32 class I small Hspwere less abundant Our results suggest heat-inducibletransactivator sustains Hsp extends Arabidopsis.

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A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance

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A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance

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