Variability in Gene Expression Patterns of Ewing Tumor Cell Lines Differing in EWS-FLI1 Fusion Type

摘要: Type 1 and type 2 EWS-FLI1 fusion products result from variation in breakpoint locations arising the t(11;22)(q24;q12) recurrent chromosomal translocation Ewing's sarcoma family tumors (EFT). Previously, studies our institution (updated present communication at a median follow-up of more than 6 years) others suggested prognostic difference for EFT patients with localized disease depending on tumor. It has been that observed clinical discrepancies different transactivation potentials various proteins. In an attempt to identify genes whose expression levels are differentially modulated by structurally transcription factors, we have used two related PCR-based subtractive approaches, cDNA representational analysis (cDNA-RDA) linker-capture subtraction (LCS) compare transcript representations pools versus cell lines. About 800 clones obtained approaches were analyzed dot blot hybridization pools. Eighty-six showing highest variability signal intensities blots further hybridized individual line RNAs Northern blots, four them additionally studied real-time quantitative PCR (RTQ-PCR). Although interindividual variations gene patterns range one- several-fold observed, no correlation specific types could be identified. Among expressed lines several previously implicated tumor growth, invasion, metastasis. data may revealed candidate composite pattern relevant biology EFT, they do not support role distinct prognosis based potentials.