Oxygen Tolerance of the H2-sensing [NiFe] Hydrogenase fromRalstonia eutrophaH16 Is Based on Limited Access of Oxygen to the Active Site
作者： Thorsten Buhrke , Oliver Lenz , Norbert Krauss , Bärbel Friedrich
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摘要: Hydrogenases, abundant proteins in the microbial world, catalyze cleavage of H2 into protons and electrons or evolution by proton reduction. Hydrogen metabolism predominantly occurs anoxic environments mediated hydrogenases, which are sensitive to inhibition oxygen. Those microorganisms, thrive oxic habitats, contain hydrogenases that operate presence We have selected H2-sensing regulatory [NiFe] hydrogenase Ralstonia eutropha H16 investigate molecular background its oxygen tolerance. Evidence is presented shape size intramolecular hydrophobic cavities leading active site crucial for insensitivity. Expansion putative gas channel site-directed mutagenesis yielded mutant derivatives oxygen, presumably because has become accessible The revealed characteristics typical standard as described Desulfovibrio gigas Allochromatium vinosum. data offer a new strategy how engineer oxygen-tolerant biotechnological application.
highwire.org参考文章(44)
Anastasios Melis, Thomas Happe, Hydrogen Production. Green Algae as a Source of Energy Plant Physiology. ,vol. 127, pp. 740- 748 ,(2001) , 10.1104/PP.010498
Boris Bleijlevens, Thorsten Buhrke, Eddy van der Linden, Bärbel Friedrich, Simon P. J. Albracht, The auxiliary protein HypX provides oxygen tolerance to the soluble [NiFe]-hydrogenase of ralstonia eutropha H16 by way of a cyanide ligand to nickel. Journal of Biological Chemistry. ,vol. 279, pp. 46686- 46691 ,(2004) , 10.1074/JBC.M406942200
Michael Bernhard, Thorsten Buhrke, Boris Bleijlevens, Antonio L. De Lacey, Victor M. Fernandez, Simon P. J. Albracht, Bärbel Friedrich, The H2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase. Journal of Biological Chemistry. ,vol. 276, pp. 15592- 15597 ,(2001) , 10.1074/JBC.M009802200
A.A. Karyakin, S.V. Morozov, E.E. Karyakina, N.A. Zorin, V.V. Perelygin, S. Cosnier, Hydrogenase electrodes for fuel cells. Biochemical Society Transactions. ,vol. 33, pp. 73- 75 ,(2005) , 10.1042/BST0330073
Thorsten Buhrke, Simone Löscher, Oliver Lenz, Eberhard Schlodder, Ingo Zebger, Lars K. Andersen, Peter Hildebrandt, Wolfram Meyer-Klaucke, Holger Dau, Bärbel Friedrich, Michael Haumann, Reduction of Unusual Iron-Sulfur Clusters in the H2-sensing Regulatory Ni-Fe Hydrogenase from Ralstonia eutropha H16 Journal of Biological Chemistry. ,vol. 280, pp. 19488- 19495 ,(2005) , 10.1074/JBC.M500601200
Sophie E. Lamle, Simon P. J. Albracht, Fraser A. Armstrong, Electrochemical potential-step investigations of the aerobic interconversions of [NiFe]-hydrogenase from Allochromatium vinosum: insights into the puzzling difference between unready and ready oxidized inactive states. Journal of the American Chemical Society. ,vol. 126, pp. 14899- 14909 ,(2004) , 10.1021/JA047939V
Baowei Chen, A. E Przybyla, An efficient site-directed mutagenesis method based on PCR. BioTechniques. ,vol. 17, pp. 657- 659 ,(1994)
Ann Wood, D P Kelly, The prokaryotes: an evolving electronic resource for the microbiological community - Springer US. ,(2002)
P M Vignais, A Colbeau, Molecular biology of microbial hydrogenases. Current Issues in Molecular Biology. ,vol. 6, pp. 159- 188 ,(2004)
Hydrogen as a Fuel : Learning from Nature CRC Press. ,(2001) , 10.1201/9780203471043