The Ets Transcription Factor EHF as a Regulator of Cornea Epithelial Cell Identity

摘要: The cornea is the clear, outermost portion of eye composed three layers: an epithelium that provides a protective barrier while allowing transmission light into eye, collagen-rich stroma, and endothelium monolayer. How development aging controlled poorly understood. Here we characterize mouse transcriptome from early embryogenesis through compare it with transcriptomes other epithelial tissues, identifying cornea-enriched genes, pathways, transcriptional regulators. Additionally, profiled defining genes enriched in these layers. Over 10,000 are differentially regulated across time course, showing dynamic expression during modest changes fewer aging. A striking transition point for gene between postnatal days 14 28 corresponds completion at level. Clustering classifies co-expressed, potentially co-regulated, biologically informative categories, including groups exhibit or stromal expression. Based on findings, loss function studies ChIP-seq, show Ets transcription factor EHF promotes fate complementary activating repressing activities. Furthermore, identify potential interactions EHF, KLF4, KLF5 promoting differentiation. These data provide insights mechanisms underlying aging, as regulator identity pointing to KLF factors fate. this comprehensive set powerful tool discovery novel regulators pathways.