MHC Genotyping by SSCP and Amplicon-Based NGS Approach in Chamois.

摘要: Genes of the major histocompatibility complex (MHC) code for cell surface proteins essential adaptive immunity. They show most outstanding genetic diversity in vertebrates, which has been connected with various fitness traits and thus long-term persistence populations. In this study, polymorphism MHC class II DRB locus was investigated chamois Single-Strand Conformation Polymorphism (SSCP)/Sanger genotyping Ion Torrent S5 next-generation sequencing (NGS). From eight identified variants 28 individuals, five had already described, three were new, undescribed alleles. With conventional SSCP/Sanger sequencing, we able to detect seven alleles, all also detected NGS. We found inconsistencies individual genotypes between two methods, mainly caused by allelic dropout method. Six out individuals falsely classified as homozygous analysis. Overall, 25% discrepancies methods. Our results that NGS technologies are better performing highly variable regions such MHC, they have a higher detection capacity, allowing more accurate description composition, is crucial evolutionary population studies.