Detection of endolithic spatial distribution in marble stone.

摘要: Summary The penetration of endolithic microorganisms, which develop to depths several millimetres or even centimetres into the stone, and diffusion their extracellular substances speeds up stone deterioration process. The aim this study was investigate, using a confocal laser scanning microscopy with double-staining, marble rock sample by observing spatial distribution quantifying volume they occupied within in order understand real impact these microorganisms on conservation monuments. Often only factors taken account biodeterioration studies regarding are spread depth penetration. Despite knowledge three-dimensional quantification volume, it is indispensable damage caused monuments. In work, we analyze stained propidium iodide Concavalin-A conjugate fluorophore Alexa Fluor 488, comparing results other techniques (SEM microscope, microphotographs polished cross-sections thin-section, PAS staining methods), An image analysis approach has also been applied. The use double shows clear evidence presence (cyanobacteria fungi) as well polymeric substance matrix architecture part sample, technique, therefore, seems very useful when applied restoration interventions monuments growth suspected.