Complete genome sequence of the incompatibility group I1 plasmid R64

摘要: Abstract A streptomycin and tetracycline resistance plasmid R64 isolated from Salmonella enterica serovar Typhimurium belongs to the incompatibility group I1 (IncI1). The DNA sequence of conjugative transfer region was described previously ( Komano et al., 2000 ). Here, we report complete genome R64. In circular double-stranded with 120,826 bp, 126 ORFs are predicted. addition, 2 6 different kinds proteins produced by translational reinitiation shufflon multiple inversions, respectively. consists five major regions: replication, drug resistance, stability, leading, regions in clockwise order. nucleotide essential for autonomous replication is completely identical that IncI1 colicinogenic ColIb-P9, an indication these two plasmids share same mechanisms copy number control. Tetracycline genes encoded transposons Tn 10 6082 , These insertion elements, IS 1133 were inserted stepwise into arsenic-resistant gene, arsA1, present region. stability leading contain various important such as parAB resD ardA psiAB or ssb maintenance, recombination reactions. When compared those other plasmids, varying levels similarity observed. It suggested genetic recombinations including site-specific rfsF -ResD system have played role diversity genomes related found exhibits highly organized structure.