Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows.

摘要: Contents To investigate the causes of occurrence and persistence porcine cystic follicles, we evaluated apoptosis proliferation follicular cells in these cysts. Apoptotic frequencies were examined by TUNEL assay expression regulators (XIAP, bax, bc1-2 caspase-3) immunohistochemistry, Western blotting real-time quantitative PCR; cell activity was PCNA immunohistochemistry vitro cultured granulosa theca cells. The low apoptotic frequency weak proliferative found follicles. Low might be associated with decreased amounts apoptotic-related factors (bax increased anti-apoptotic (XIAP bcl-2) Significantly lower detected from lesser PCNA-positive Our results indicate that programmed death system altered disorder between responsible for maintaining a static condition without degeneration, which leads to long-term These findings provide important novel insights into pathogenesis cysts sows.