The ATP-dependent translocation of phosphatidylserine to the mitochondria is a process that is restricted to the autologous organelle.

摘要: The general properties of phosphatidylserine synthesis and translocation to the inner mitochondrial membrane which occur in both intact permeabilized cells are now shown that disrupted by shearing. In requires ATP is blocked preventing Ca2+ sequestration. nascent mitochondria also cells. Using it possible test whether restricted autologous acceptors or with heterologous acceptors. To examine nature transport process, a donor acceptor population were prepared. poisoned hydroxylamine irreversibly inactivates decarboxylase will prevent conversion phosphatidylethanolamine. not treated so any generated cell reached would be decarboxylated form Disrupted pulse labeled [3H]serine for 40 min permit [3H] phosphatidylserine, then further incorporation radiolabel was arrested chelating Ca2+. When prelabeled mixed latter failed decarboxylate phosphatidylserine. control experiments addition hydroxylamine-poisoned did alter transfer present unpoisoned These results indicate ATP-dependent process organelle.