Occurrence and community composition of fast-growing Mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons

摘要: Fast-growing mycobacteria are considered essential members of the polycyclic aromatic hydrocarbons (PAH) degrading bacterial community in PAH-contaminated soils. To study natural role and diversity Mycobacterium contaminated soils, a culture-independent fingerprinting method based on PCR combined with denaturing gradient gel electrophoresis (DGGE) was developed. New primers were selected which specifically targeted 16S rRNA genes fast-growing mycobacteria, single-band DGGE profiles amplicons obtained for most strains tested. Strains belonging to same species revealed identical fingerprints, cases, but not all, these fingerprints typical one species, allowing partial differentiation between community. inoculated soil detected detection limit 10(6) CFU g(-1) using new primer set as such, or approximately 10(2) nested approach combining eubacterial specific primers. Using PCR-DGGE method, different could be individually recognized mixed This used rapidly assess structure several soils diverse origin overall contamination profiles, pollution concentrations chemical-physical characteristics. In non-contaminated soil, recovered gene sequence did match previous described PAH-degrading strains. closely related such frederiksbergense austroafricanum, that known include capacities. Interestingly, M. tusciae sequences, so far reported relation biodegradation PAHs, all (C) 2004 Federation European Microbiological Societies. Published by Elsevier B.V. All rights reserved.