Potent Synergism of the Combination of Fluconazole and Cyclosporine in Candida albicans

摘要: Several types of drugs currently used in clinical practice were screened vitro for their potentiation the antifungal effect fungistatic agent fluconazole (FLC) on Candida albicans. These included inhibitors multidrug efflux transporters, antimicrobial agents, and membrane-active compounds with no activity, such as antiarrhythmic proton pump inhibitors, platelet aggregation inhibitors. Among tested an agar disk diffusion assay, cyclosporine (Cy), which had intrinsic showed a potent combination FLC. In checkerboard microtiter plate format, however, it was observed that MIC FLC, classically defined by NCCLS recommendations, unchanged when FLC Cy combined. Nevertheless, if different reading endpoint corresponding to minimal fungicidal concentration needed decrease viable counts at least 3 logs comparison growth control chosen, synergistic (fractional inhibitory index 32 >10 μg/ml, respectively, decreased 0.5 0.625 μg/ml two The μg per ml supra-MICs resulted time-kill curve experiments. This or fungistatic, depending C. albicans strain used. Since effective is achievable vivo, this represents attractive perspective development new management strategies candidiasis.