The receptor of an oyster juice-borne coliphage OJ367 in the outer membrane of Salmonella derby.
作者： Yuan Tih Ko
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摘要: The objective of this study was to identify the receptor OJ367, an oyster juice-borne bacteriophage, in Salmonella derby ATCC 6960. crude outer membrane (OM) fraction prepared and examined from total cell envelope (TCE) by differential extraction with N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES)-MgCl2 then Triton-HEPES-ethylenediamine tetra-acetic buffers. OM proteins (Omps) were isolated diethylaminoethyl column chromatography screen for activity. A 45-kDa protein belonging a minor Omp species, phage neutralization ability, eluted homogeneous form. It non-peptidoglycan-associated which digestible trypsin. Lipopolysaccharide had no influence on its activity when coexistent fractions. An S. mutant resistant lysis OJ367 isolated. not only showed decreased vitro TCE tested but altered profile. This implied that is involved as coliphage binding; however, role affected expression other Omps.
参考文章(19)
T J Chai, J Foulds, Two bacteriophages which utilize a new Escherichia coli major outer membrane protein as part of their receptor. Journal of Bacteriology. ,vol. 135, pp. 164- 170 ,(1978) , 10.1128/JB.135.1.164-170.1978
Hiroko TOKUNAGA, Masao TOKUNAGA, Taiji NAKAE, Characterization of porins from the outer membrane of Salmonella typhimurium. 1. Chemical analysis. FEBS Journal. ,vol. 95, pp. 433- 439 ,(1979) , 10.1111/J.1432-1033.1979.TB12982.X
T Nakae, H Nikaido, Outer membrane as a diffusion barrier in Salmonella typhimurium. Penetration of oligo- and polysaccharides into isolated outer membrane vesicles and cells with degraded peptidoglycan layer. Journal of Biological Chemistry. ,vol. 250, pp. 7359- 7365 ,(1975) , 10.1016/S0021-9258(19)40952-6
T J Chai, J Foulds, Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane. Journal of Bacteriology. ,vol. 130, pp. 781- 786 ,(1977) , 10.1128/JB.130.2.781-786.1977
D B Datta, B Arden, U Henning, Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors. Journal of Bacteriology. ,vol. 131, pp. 821- 829 ,(1977) , 10.1128/JB.131.3.821-829.1977
R Morona, U Henning, Host range mutants of bacteriophage Ox2 can use two different outer membrane proteins of Escherichia coli K-12 as receptors. Journal of Bacteriology. ,vol. 159, pp. 579- 582 ,(1984) , 10.1128/JB.159.2.579-582.1984
Sohair Farid Sabet, Carl A. Schnaitman, Purification and Properties of the Colicin E3 Receptor of Escherichia coli Journal of Biological Chemistry. ,vol. 248, pp. 1797- 1806 ,(1973) , 10.1016/S0021-9258(19)44261-0
Jurg P. Rosenbusch, Characterization of the Major Envelope Protein from Escherichia coli Journal of Biological Chemistry. ,vol. 249, pp. 8019- 8029 ,(1974) , 10.1016/S0021-9258(19)42066-8
T Nogami, S Mizushima, Outer membrane porins are important in maintenance of the surface structure of Escherichia coli cells. Journal of Bacteriology. ,vol. 156, pp. 402- 408 ,(1983) , 10.1128/JB.156.1.402-408.1983
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)