Generation and maintenance of autoantigen-specific CD8(+) T cell clones isolated from NOD mice.

摘要: Abstract The non-obese diabetic (NOD) mouse develops insulin dependent diabetes mellitus (IDDM) spontaneously with a higher incidence in females than males. There are many similarities to the human disease, making it an ideal model. Our group is examining role that CD4 + and CD8 T cells play IDDM NOD mouse, as known both cell subsets required for onset of disease. Although has autoimmune etiology, initial triggering event unknown autoantigen involved not been identified. This investigation focussed on one potential autoantigens involved, enzyme glutamic acid decarboxylase (GAD). We raised GAD peptide-specific by immunising mice peptide alongside irrelevant induced response. In order maintain these specific vitro generate clones, was found antibodies MHC class II molecules needed be included culture medium. paper outlines methods we employed vitro.