Thin-layer chromatographic screening methods for histamine in tuna fish

摘要: Abstract Twelve solvent systems were tested for their ability to separate histamine and histidine on a variety of thin-layer coatings. The best solvent—adsorbent were: chloroform—methanol—ammonia (2:2:1), methanol—ammonia (20:1), acetone—ammonia (95:5), double development with (a) n -butanol—acetone—water (2:2:1) (b) (12:7:1), all siliga-gel layers. Ninhydrin was used as the visualization reagent. These four then evaluated potential use rapid screening procedures in detection possibly deleterious levels tuna fish. Successful separation from other ninhydrin-positive components methanolic fish extracts achieved systems. A sample lot implicated human illness found have level considerably higher than purchased local retail outlet or an extract spiked considered be threshold value toxicity symptoms. (20:1) systems, silica-gel plates, are most promising preliminary histamine.