Chapter 15 Serial Cultivation of Normal Human Epidermal Keratinocytes

摘要: Publisher Summary This chapter discusses the serial cultivation of normal human epidermal keratinocytes. Fibroblasts can be grown from almost any explanted tissue and serially passaged even cloned low-density platings. Keratinocyte colonies are initiated by plating a single-cell suspension keratinocytes with 3T3 feeder cells in usual way such that less than 300 arise. Newborn foreskin is convenient source possessing high-proliferative capacity culture. Trypsin alone sufficient to completely disaggregate this single yield about 3 million per square centimeter surface. The attach directly culture vessel surface push away as expand. In secondary subsequent cultures, however, do not assume epithelioid morphology during early stage colony initiation absence hydrocortisone, although colony-forming efficiency unaffected. Cells growing hydrocortisone eventually organize into time have reached size 100 cells. expression tissue-specific structure function cultured discussed chapter.