CYP2A6 genotyping methods and strategies using real-time and end point PCR platforms

摘要: CYP2A6 genotyping is of clinical importance--CYP2A6 gene variants influence nicotine metabolism and are associated with dependence, cigarettes per day, smoking cessation the risk for tobacco-associated cancers. also therapeutic drugs, such as anticancer agents, tegafur letrozole. Over years, methods have evolved to incorporate novel circumvent errors resulting from high degree homology between neighboring CYP2A genes. Herein, strategies described commonly genotyped functionally significant alleles including SNPs, small insertions/deletions more complex structural variants. The presented utilize higher throughput SYBR green real-time PCR technology in addition standard thermocycling.